Immunofluorescence (IF) | Wax-it Histology Services Inc.

IF multiplexing

Multiplex_CD8a (Green, AF488), HepBC (Red, AF594) HepBS (Magenta, AF647) CD11c (Yellow, AF750)

A SHOWCASE OF MULTIPLEXING

Explore our collection of Multiplex Immunofluorescence images showcasing different signals across various tissue types. Each image highlights the detection and localization of wide variety of antigens in different types of cell preparations. Gain insight into our expertise in advanced immunofluorescence techniques and imaging capabilities.

Stain Collection

Mouse Eye - DAPI – Blue Zonula Occludens 1 - Green Thrombospondin 1 – Orange IBA1 – Magenta. 63x

Mouse Eye – DAPI – Blue Zonula Occludens 1 – Green Thrombospondin 1 – Orange IBA1 – Magenta. 63x

Image of immunofluorescence stained mouse brain tissue - FMRP (Green), NeuN (Red) and DAPI (Blue)

FMRP (Green), NeuN (Red) and DAPI (Blue) – Mouse Brain

Mouse brain - GFAP (Green), IBA1 (Red), Aβ plaques and nuclei (Blue

Mouse brain – GFAP (Green), IBA1 (Red), Aβ plaques and nuclei (Blue)

Brain tissue visualized by IF

Brain- CD31 (Green), NeuN (Red), GFAP (Orange), GFAP (Blue)

Image of immunofluorescence stained mouse spinal cord tissue - NeuN (Yellow), ChAT (Red) and DAPI (Blue)

NeuN (Yellow), ChAT (Red) and DAPI (Blue) – Mouse Spinal Cord

Image of mouse spinal cord tissue immunofluorescence stained for CD8A with DAPI

DAPI-CD8A – Mouse Spinal Cord

IF stain of mouse eye - DAPI – Blue Zonula Occludens 1 - Green Thrombospondin 1 – Orange IBA1 – Magenta - 20x

Mouse Eye – DAPI – Blue Zonula Occludens 1 – Green Thrombospondin 1 – Orange IBA1 – Magenta 20x

IF multiplex - CD8a (Green, AF488), HepBC (Red, AF594) HepBS (Magenta, AF647) CD11c (Yellow, AF750)

Multiplex_CD8a (Green, AF488), HepBC (Red, AF594) HepBS (Magenta, AF647) CD11c (Yellow, AF750) (1) (2)

Image of immunofluorescence stained mouse spinal cord tissue - iNOS (Red) and DAPI (Blue).

iNOS (Red) and DAPI (Blue) – Mouse Spinal Cord

Image of immunofluorescence stained mouse liver tissue - F4/80 (Green) and DAPI (Blue)

F4/80 (Green) and DAPI (Blue) – Mouse Liver

Mouse Eye - DAPI – Blue Zonula Occludens 1 - Green Thrombospondin 1 – Orange IBA1 – Magenta. 63x (1)

Mouse Eye – DAPI – Blue Zonula Occludens 1 – Green Thrombospondin 1 – Orange IBA1 – Magenta. 63x (1)

Image of immunofluorescence stained mouse spinal cord tissue - CD8a (Red) and DAPI (Blue)

CD8a (Red) and DAPI (Blue) – Mouse Spinal Cord

Image of immunofluorescence stained mouse eye tissue - MerTK (Red) and DAPI (Blue).

MerTK (Red) and DAPI (Blue) – Mouse Eye

Fluoro-jade B staining following hemorrhagic stroke

Fluoro-jade B staining following hemorrhagic stroke

Image of immunofluorescence stained mouse liver tissue - F4/80 (Green) and DAPI (Blue)

F4/80 (Green) and DAPI (Blue) – Mouse Liver

Image of immunofluorescence stained mouse ear tissue - CD68 (Red) and DAPI (Blue)

CD68 (Red) and DAPI (Blue) – Mouse Ear

Image of immunofluorescence stained mouse ear tissue - CD68 (Red) and DAPI (Blue)

CD68 (Red) and DAPI (Blue) – Mouse Ear

LAMP1 (Green) and NeuN (Red) - Mouse Brain 20x

LAMP1 (Green) and NeuN (Red) – Mouse Brain 20x

Iba-1, P2X2, HMGB1, & ZO1 - Glioblastoma

Iba-1, P2X2, HMGB1, & ZO1 – Glioblastoma

HSP47 & p53 - Fibrotic liver

HSP47 & p53 – Fibrotic liver

E-cadherin - Colon

E-cadherin – Colon

E-cadherin - Colon

E-cadherin – Colon

CD68 (Red) - Mouse Ear 40x

CD68 (Red) – Mouse Ear 40x

Vimentin - Hepatocellular carcinoma

Vimentin – Hepatocellular carcinoma

STAT3a - Lung cancer

STAT3a – Lung cancer