Western Immunoblot

Western Immunoblot using Formalin Fixed and Paraffin Embedded Tissues

As a result of years of dedication to discoveries, Wax-it unveils transformative methods for protein detection and identification within complex mixtures. Beyond mere identification, Western Blotting provides invaluable insights into protein size and quantity, shedding light on their expression levels within tissues.

We have pioneered advanced methods and techniques to conduct Western Immunoblotting from formalin-fixed tissue and paraffin-embedded tissue (FFPE), opening a range of possibilities in groundbreaking preclinical research through protein quantification. Join us on this journey of exploration to uncover proteins in different types of tissue samples.

Comprehensive Western Blot Services

We provide a full suite of Western Blot services designed to guide our clients through every step of the process:

Expert Sample Preparation: Tailored guidance on sample preparation for optimal protein extraction and solubilization, ensuring that each project's unique needs are met.
Gel Electrophoresis: Customized protocols that allow for precise protein separation, enabling accurate analysis of protein size and abundance.
Advanced Membrane Transfer Techniques: Techniques designed to ensure efficient protein transfer to membranes while preserving protein integrity and minimizing background noise.
Antibody Selection and Optimization: Assistance in selecting and optimizing antibodies to ensure high specificity and sensitivity for detecting target proteins.
Chemiluminescent Detection: Utilization of chemiluminescence to provide flexibility and enhanced sensitivity for protein visualization and quantification.

Advanced Imaging and Analysis with the iBright Imaging System

Our Western Blot capabilities are enhanced by the iBright Imaging System (CL750 model), which supports high-quality imaging and detailed analysis of Western blots and protein gels:

High-Quality Imaging: Captures publication-quality images, essential for sharing and publication of research findings.
Comprehensive Data Analysis: Features advanced tools for densitometry, quantitation, and normalization, enabling researchers to perform detailed analysis directly on the system.
Report Generation: The iBright system can generate comprehensive reports that include all data obtained from the analysis, providing a convenient overview of results for further review or publication.

Image Analysis and Data Interpretation

At Wax-it, our Western Immunoblot service is complemented by extensive data analysis capabilities, including:

Protein Band Quantification: Quantitative analysis of protein bands to measure and compare protein levels.
Normalization and Statistical Analysis: Methods to normalize protein levels against controls and perform statistical analysis to ensure reliable and reproducible results.

Visual Documentation

At Wax-it, our Western Immunoblot service is complemented by extensive data analysis capabilities, including:

At Wax-it Histology Services, our goal is to provide high-level support and reliable results for researchers conducting Western Blot analysis. By offering expert consultation, precise analytical techniques, and advanced imaging capabilities, we help our clients achieve insightful, quantifiable research outcomes that propel scientific discovery forward.